Access to the resources available at the UWPR is through collaborations with Resource members listed on our Contacts page. We strongly recommend that you discuss your specific proteomics project and the preparation of your protein samples with the Resource members prior to preparing samples! Check out the sample preparation guidelines on our Methods page.
The UWPR is offering analytical and instructional expertise in state of the art mass spectrometric techniques and data analysis with the primary aim of providing UW investigators with "hands-on" training and access to contemporary resources needed to develop and meet their proteomics research interests.
In order to use the resources, you will have to undergo the appropriate training and follow all the usage guidelines. The level of instruction is such that following completion of the initial training (usually 2 to 4 hrs) and several periods of supervised use, the researcher may exercise independent, unattended use of an instrument with instructor approval. The training is instrument specific and is provided during the signed up instrument time and has to be coordinated with UWPR staff.
The UWPR is operated as a Cost Center through which institutional users contribute to the upkeep of the resource by means of a recharge system. Rates are based on the operating costs and individual instrument usage. To keep the operating costs low, our collaborators must supply all the consumables needed for sample preparation and mass spec analysis (including LC columns, vials etc, as per usage guidelines).
All rates are reviewed and approved by the University of Washington Administration and are subject to change without notice. To view the current fee schedule, register and log into to the Internal Proteomics Resource website using the login form above.
Access to UWPR resources and services
To initiate a new collaboration, register/log in using the login form above. On the Welcome page click on the link "Start new collaboration" and submit a detailed project description.
Note UW emplyees using UW budget numbers for billing can choose between self service (do the LC-MS analyses yourself) or Fee-For-Service (LC-MS analyses done by UWPR personnel). When submitting your project description make sure you check "Mass spec analysis by UWPR personnel" NO for self service and YES for Fee-For-Service.
All other collaborators are limited to Fee-For-Service.
Download the Self service guidelines.
After submitting the project details and billing information, coordinate time to drop off your "mass spec" ready samples. Feel free to contact us if you need help with project design/sample prep procedures. With every set of samples you also need to submit a Sample Submission Form which can be downloaded here. Once we receive both the samples and the submission form, UWPR personnel will schedule instrument time needed to analyze your samples.
UWPR personnel can help you with your data analysis needs as well.
The cost is based on the instrument time you signed up for and will be invoiced the first business day in the following month. Cancellation is 48hr prior to your start time. Any time that is not cancelled will be charged.
For Fee-For-Service the cost is based on the time it take to analyze your samples plus a min 2hr setup time. The Sample Submission Form can help estimate the time needed to analyze your samples.
The current rates can be downloaded from the "Welcome page" when you login. The billing cycle is monthly.
It is our policy that key collaborator(s) within the UWPR share in co-authorship of any manuscripts that are the result of work performed at the Resource. Minimally, the Proteomics Resource should always be acknowledged as follows in publications:
This work is supported in part by the University of Washington's Proteomics Resource (UWPR95794).
Here are the ABRF guidelines for authorship for personnel working in facilities such as the UWPR.
Both short term and long term storage of the acquired files is the sole responsibility of the users of the Resource. The UWPR will strive to maintain (but not guarantee) copies of acquired raw data files for up to one year of the acquisition date. After that time, the files will be purged/deleted from our storage systems.
Here is a list of other local fee-for-service facilities available to service your mass spec or proteomics needs.