Access to the resources available at the UWPR is through collaborations with Resource members listed on our Contacts page. We strongly recommend that you discuss your specific proteomics project and the preparation of your protein samples with the Resource members prior to preparing samples! Check out the sample preparation guidelines on our Methods page.

The UWPR is offering analytical and instructional expertise in state of the art mass spectrometric techniques and data analysis with the primary aim of providing UW investigators with "hands-on" training and access to contemporary resources needed to develop and meet their proteomics research interests.

In order to use the resources, you will have to undergo the appropriate training and follow all the usage guidelines. The level of instruction is such that following completion of the initial training (usually 2 to 4 hrs) and several periods of supervised use, the researcher may exercise independent, unattended use of an instrument with instructor approval. The training is instrument specific and is provided during the signed up instrument time and has to be coordinated with UWPR staff.

The UWPR is operated as a Cost Center through which institutional users contribute to the upkeep of the resource by means of a recharge system. Rates are based on the operating costs and individual instrument usage. To keep the operating costs low, our collaborators must supply all the consumables needed for sample preparation and mass spec analysis (including LC columns, vials etc, as per usage guidelines).

All rates are reviewed and approved by the University of Washington Administration and are subject to change without notice. To view the current fee schedule, register and log into to the Internal Proteomics Resource website using the login form above.

Access to UWPR resources and services

To initiate a new collaboration, register/log in using the login form above. On the Welcome page click on the link "Start new collaboration" and submit a detailed project description.
Note UW emplyees using UW budget numbers for billing can choose between self service (do the LC-MS analyses yourself) or Fee-For-Service (LC-MS analyses done by UWPR personnel). When submitting your project description make sure you check "Mass spec analysis by UWPR personnel" NO for self service and YES for Fee-For-Service.
All other collaborators are limited to Fee-For-Service.

Self Service:

Download the Self service guidelines.

• After submitting the project details and billing information, you'll have to request sign up privileges via email. Once we grant you access you will be able to schedule instrument time up to max 20 days (480hrs).
  
  
• Do NOT block instrument time unnecessarily! Your self sign up rights can and will be revoked.
  
  
• There is a non-refundable fee for singing up. The fee is a percentage of the total cost and will be billed even if you cancel your instrument time.
• There is a 48 hrs cancellation time, i.e. you can cancel instrument time up to 48hr before the start time, after that the system will no longer allow you to make changes. So it's very simple: you will be charged for all the sign up fees and the instrument time you cannot cancel yourself.
• Make sure to coordinate with UWPR personnel to get access to the facility and schedule instruction time if training is needed.
• Start and end times are strictly enforced. If you start early or don't finish, clean up and return the key card on time you will be charged extra time at the hourly rate (always rounding up to the next full hour).
• Instrument start times are 10 am and 2 pm (Note UWPR personnel may need time to prep the instrument at the beginning of your start time)
• Instrument end times are 10 am, 2 pm and 6 pm.
• If you finish early and clean up/return the key card before the next time block starts, unused instrument time will be backed out to the end of the last acquisition (always rounding up to the next full hour), but no more than back to the end of the previous time block. The sign-up fee will not be backed out:
  • e.g. if you are signed up until 10 am, time from the end of your last acquisition (always rounding up to the next full hour) will be backed out, maximum back to 6 pm the day before.
  • if you are signed up until 6pm, time will be backed out to max 2 pm that same day
  • if you are signed up until 2pm, time will be backed out to max 10 am that same day
  • So for example, if you wish to sign up for the whole weekend (e.g. until Mon 10am), so you don't have to cleanup during the weekend, and your last run finishes e.g. Saturday morning, you will be charged until Sunday 6pm.
• If you can't make it in to clean up and ask UWPR personnel to clean up for you, you will be charged the full time block (no time will be backed out).
• If you sign up into the weekend, be prepared to come in and clean up your stuff during the weekend, in case someone signs up to use the instrument.
• You will have to provide your own consumables (columns and autosampler vials),
  click here to download a list of LC-MS commonly used consumables (xls)
• UWPR personnel can help you with your data analysis needs as well, please contact us to discuss details.

Fee-For-Service:
After submitting the project details and billing information, coordinate time to drop off your "mass spec" ready samples. Feel free to contact us if you need help with project design/sample prep procedures. With every set of samples you also need to submit a Sample Submission Form which can be downloaded here. Once we receive both the samples and the submission form, UWPR personnel will schedule instrument time needed to analyze your samples. There is a non-refundable fee for singing up. The fee is a percentage of the total cost and will be billed even if you cancel your instrument time.
UWPR personnel can help you with your data analysis needs as well.

Billing
The cost is based on the sign up fee and the instrument time you signed up for and will be invoiced the first business day the month following the signed up time.
The sing-up fee can NOT be cancelled. Instrument time cancellation is 48hr prior to the start time of the time block you wish to cancel. Any time that is not cancelled will be charged.
For Fee-For-Service the cost is based on the time it take to analyze your samples plus a min 2hr setup time. The Sample Submission Form can help estimate the time needed to analyze your samples.
The current rates can be downloaded from the "Welcome page" when you login. The billing cycle is monthly.

Publication/Citation
It is our policy that key collaborator(s) within the UWPR share in co-authorship of any manuscripts that are the result of work performed at the Resource. Minimally, the Proteomics Resource should always be acknowledged as follows in publications:
This work is supported in part by the University of Washington's Proteomics Resource (UWPR95794).

Here are the ABRF guidelines for authorship for personnel working in facilities such as the UWPR.

Data retention
Both short term and long term storage of the acquired files is the sole responsibility of the users of the Resource. The UWPR will strive to maintain (but not guarantee) copies of acquired raw data files for up to one year of the acquisition date. After that time, the files will be purged/deleted from our storage systems.

Chorus offers very affordable and reliable cloud data storage and analysis. Check it out go to Chorus

Here is a list of other local fee-for-service facilities available to service your mass spec or proteomics needs.