Access to the resources available at the UWPR is through collaborations with Resource members. We strongly recommend that you discuss your specific proteomics project and the preparation of your protein samples with the Resource members prior to preparing samples! We will post some basic sample preparation guidelines on our Tools page in the near future, so please check back soon.
The UWPR is offering analytical and instructional expertise in state of the art mass spectrometric techniques and data analysis with the primary aim of providing investigators with "hands-on" training and access to contemporary resources needed to develop and meet their proteomics research interests.
In order to use the resources, you will have to undergo the appropriate training and follow all the usage guidelines. The level of instruction is such that following completion of the initial training (usually 2 to 4 hrs) and several periods of supervised use, the researcher may exercise independent, unattended use of an instrument with instructor approval. The training is instrument specific and is provided during the signed up instrument time and has to be coordinated with UWPR staff.
The UWPR is operated as a Cost Center through which institutional users (academic, non-profit and corporate) contribute to the upkeep of the resource by means of a recharge system. Rates are based on the operating costs and individual instrument usage. To keep the operating costs low, our collaborators must supply all the consumables needed for sample preparation and mass spec analysis (including LC columns, vials etc, as per usage guidelines).
All rates are reviewed and approved by the University of Washington Administration and are subject to change. To view the current fee schedule, register and log into to the informatics abstract and dissemination platform.
To initiate a new collaboration, submit a detailed project description via the informatics abstract and dissemination platform. After submitting the project details and billing information, you will be able to schedule instrument time. But make sure to coordinate with UWPR personnel to get access to the facility and schedule instruction time if training is needed.
The UWPR will be supporting a limited number of projects by granting access to resources at no charge. The intent is to support small scale projects (max. 5 days of instrument time), e.g. to produce preliminary data for a grant proposal. The mechanism of proposing a UWPR supported collaboration is through dialogue with a Resource member and ultimately the submission of an abstract. If you are interested in a supported collaboration, please interact with an appropriate UWPR member (specifically Drs. Bruce, Goodlett, Heinecke, Hoofnagle, MacCoss, Ong, Villen, Wolf-Yaldin) and pending consent, register and submit a collaboration request via the informatics abstract and dissemination platform.
Note that the UWPR is focused on supporting only the best science, from novel biology to cutting edge technology. Only abstracts proposing generation of novel biological data and/or novel use of existing technology will be pursued. UWPR supported collaboration requests are reviewed to determine suitability and acceptance of the proposed project. The review process can be lengthy, upwards of one month, so please plan ahead.
SLU Proteomic Affiliates
Those University of Washington laboratories with extensive expertise in proteomics data generation may petition the Resource Advisory Group for affiliate membership. PI's from these laboratories should submit a one page bullet summary of their laboratory expertise along with their NIH biosketch as well as with similar material from the expert in their laboratory who will conduct the experiments. We expect that this individual will have extensive prior training in the field of mass spectrometry based proteomics. Having these items, the Resource Advisory Group will consider the request at the next regularly scheduled meeting. If approved the affiliate laboratory will be able to submit abstracts directly describing the proposed work for review.
All investigators performing projects at the UWPR are asked to submit project updates, including publications, to the UWPR advisory committee. Periodically the UWPR will be sending reminders to submit a progress report. This project summary, can be brief in nature (no more than a page in length), should highlight the scientific progress made as a result of the UWPR collaboration. A manuscript or a poster can suffice.
It is our policy that key collaborator(s) within the UWPR share in co-authorship of any manuscripts that are the result of work performed at the Resource. Minimally, the Proteomics Resource should be acknowledged as follows in publications: This work is supported in part by the University of Washington's Proteomics Resource (UWPR95794).
Here are the ABRF guidelines for authorship for personnel working in facilities such as the UWPR.
Both short term and long term storage of the acquired files is the sole responsibility of the users of the Resource. The UWPR will strive to maintain (but not guarantee) copies of acquired raw data files for up to one year of the acquisition date. After that time, the files will be purged/deleted from our storage systems.
Here is a list of local fee-for-service facilities available to service your proteomics needs.