Proteomics Protocols Overview

Proteomics Workflow: click on the links below to find related protocols, methods and references

Click here to find protocols related to protein extraction from tissues, cells, body fluids etc, as well as protein separation and purification protocols.
Protein quantification methods like SILAC and cross linking methods for structural analysis are here too.
Click here to find protocols related to protein separation.
Cell fractionation, HPLC/FPLC, IP, etc

Click here to find protocols related to protein digestion.
Labeling protocols for peptide quantification like iTRAQ and TMT are here too.

Click here for peptide separation and cleanup, SCX, avidin, IMAC, SPE etc methods

Click here for LC-MS methods: column packing, nano HPLC, MS methods.

Click here for data search and analysis tools.