This information on this page is specific to the Univ. of Washington's flavor of SEQUEST. Which means there are parameter entries here that are not supported by other versions such as the commercial version distributed by Thermo. Do not attempt to use the params files found with non-UW versions of SEQUEST.

A sequest.params file is the file which specifies the search parameters for a SEQUEST ms/ms database search. This includes things like which sequence database to search against, enzyme specificity, and modifications.

Although these parameters don't change or get added/updated often, please see each SEQUEST release page for the parameters supported by each version.