ISB 18 Standard Protein Mix Database

A mixture of tryptic digests of 18 proteins, named the ISB standard protein mix, using 8 different mass spectrometers. These include linear and 3D ion traps, two quadrupole time-of-flight platforms (qq-TOF), and two MALDI-TOF−TOF platforms. The resulting data set consists of over 1.1 million spectra in 150+ replicate runs on the mass spectrometers. These files were originally hosted at http://regis-web.systemsbiology.net/PublicDatasets/; unfortunately this server is no longer online hence they're made available here.

Publication: PubMed (PDF).

The native mass spectrometry files (Thermo raw) and mzXML conversions of these raw files are available for download. As noted in the table below, there are 6 dataset mixes available (curiously labeled 1, 2, 3, 4, 7, 8).

If you're interested in any of this data, send me (engj @uw.edu) or anyone else at the UWPR an email and we will supply you a download link. Let us know if there's a particular mix you're interested in or if you would like access to all of the files. These files are not available/linked here for direct download to avoid the constant download by data crawlers.