ISB 18 Standard Protein Mix Database

A mixture of tryptic digests of 18 proteins, named the "ISB standard protein mix", using 8 different mass spectrometers. These include linear and 3D ion traps, two quadrupole time-of-flight platforms (qq-TOF), and two MALDI-TOF−TOF platforms. The resulting data set consists of over 1.1 million spectra in 150+ replicate runs on the mass spectrometers. These files were originally hosted at http://regis-web.systemsbiology.net/PublicDatasets/; unfortunately this server is no longer online hence they're made available here. https://pubmed.ncbi.nlm.nih.gov/17711323/ (PDF).

The native mass spectrometry files (Thermo raw) and mzXML conversions of these raw files are available for download. As noted in the table below, there are 6 dataset mixes available (curiously labeled 1, 2, 3, 4, 7, 8).

If you're interested in any of this data, send me or anyone else at the UWPR an email and we will supply you a download link. Let me/us know if there's a particular mix you're interested in or if you would like access to all of the files. These files are not available/linked here for direct download to avoid the constant download by data crawlers.