LTQ-FT Ultra™

The LTQ FT Ultra system is a fully integrated hydrid mass spectrometer consisting of a Linear Ion Trap Mass spectrometer, LTQ, combined with a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer, with a 7 T actively shielded superconducting magnet. The novel ICR cell design, the Ultra Cell produces enhanced sensitivity and greater dynamic range. The linear excitation characteristics of the cell allow larger cyclotron radii which significantly reduces space charge effects. These performance gains are achieved without the costs and difficulties associated with a higher field magnet. In the ECD method, multiply charged ions are irradiated with low energy electrons produced by an emitter cathode located behind the ICR cell. Upon capture of electrons, reduced radical cations [M+nH](n-1)+· are generated which dissociate by fast and facile fragmentation of the N-Ca bond of the peptide chain, producing mainly c and z· fragment ions. Due to the nature of the ECD mechanism, co- and post-translational modifications (PTMs) such as phosphorylation, O- and N-linked glycosylation, and sulfation are preserved, allowing site-specific analyses. InfraRed MultiPhoton Dissociation (IRMPD) occurs in the ICR cell, and provides structural and peptide-sequencing information. For peptides, b and y ions are generated after a short irradiation laser pulse. IRMPD can also be used to study structural information of organic compounds.

2/5/07 Installed
10/5/07 Upgrade to Ultra cell

    Mass range
  • 50 to 4,000 Da (FT detection)
  • 50 to 2,000 Da (Ion Trap detection)
  • 100,000 at m/z 400 at 1 s per scan (broadband mode)
  • > 750,000 at m/z 400 at slower scan repetition rates (broadband mode)
    Mass accuracy
  • < 1.2 ppm RMS error with external calibration (using the calibration range and settings only)
  • < 1 ppm RMS error with internal calibration
  • Attomole range for peptides
  • Intrascan dynamic range: > 4,000
    Available scan functions
  • Collision induced dissociation (CID)
  • Pulsed Q collision induced dissociation (PQD)
  • Electron capture dissociation (ECD)
  • Infrared multi photon dissociation (IRMPD) (SYNRAD firestar series V20, Model FSV20SFB, 20 Watts continuous power output, Wavelength 10.57-10.63 microns)