TSQ Altis
The TSQ Altis, a triple quadrupole mass spectrometer, offers improved Active Ion Management (AIMâ„¢) technology, segmented quadrupoles, novel electron multipliers, and enhanced ion transmission tubes.
Visit the Thermo TSQ Altis website for more information and for some nice animations
TSQ Altis (TSQ-A-10306 / UW tag 2033410), Installed 10/16/2018
Specifications
Back to Top- Mass range
- m/z 5 to 2000 Da
- 600 SRMs/sec
- Selectivity 0.2 Da FWHM for high resolutions SRM
- Sensitivity 500 000 : 1
- Available scan functions
- Collision induced dissociation (CID)
- 3000 times SRMs (T-SRMs)
- QED-MS/MS - Simultaneous quantitation and structural confirmation
- iSRM - intelligent SRM
Instrument control software
Back to TopInstrument calibrations
Back to Top- This file summarizes calibration solutions and calibration tips Calibrations (.xlsx)
Collision Energy Calculation
Back to TopFor collision energy calculation check out our MRM/SRM overview page.
Base Collision Energy (CE) Equations TSQ Altis (based on template method from Thermo):
- TSQ Altis (1.5 mTorr) 2 + peptide: CE = 0.0339 x m/z + 2.3398
- TSQ Altis (1.5 mTorr) 3 + peptide: CE = 0.0295 x m/z + 1.4831
HPLC setup
Back to Topthe following documents are guidelines to setup the capillary trap/column on our LC-MS systems, this is intended as a supplement to the hands on training required to use the UWPR instruments.
Peptide Standards
Back to Top- Simple peptide standards for LC-MS (xlsx)
- Skyline AngioNeuro_QQQ_SRM (zip)
- Skyline Promega 6x5_LCMS_Standard_Hela_QQQ_SRM (zip)
- Skyline for TSQ Altis method template "PRTC Hela" SRM (zip)
- Skyline for TSQ Altis method template "PRTC heavy" SRM (zip)
- Skyline AngioNeuro_Orbitrap_PRM (zip)
- Skyline Promega 6x5_LCMS_Standard_Orbitrap_PRM (zip)
- Skyline Promega 6x5_LCMS_Standard_Hela_Orbitrap_PRM (zip)
- Skyline Sigma MSQC1_Orbitrap_PRM (zip)
- Skyline Hela_Orbitrap_PRM (zip)