Protocols & Methods

Step-by-step bench protocols used at the UWPR

These protocols cover sample preparation, protein digestion, peptide fractionation, and capillary column preparation, available as pages on this site or as downloadable PDF documents.

Sample Preparation

• Cell Lysis and Subcellular Fractionation
• Plasma and Serum Protein Preparation
• In-Gel Digestion

Digestion protocols

• FASP Protocol
• RapiGest SF Surfactant Protocol
• PPS Silent Surfactant Protocol
• Protein digestion protocols (pdf): several digestion protocols including trypsin and LysC

Peptide Fractionation

• Peptide fractionation and cleanup (pdf): several peptide cleanup and separation protocols including C18, SCX, and avidin

Column Preparation

• Packing capillary columns (pdf)
• Making a fritted fused silica (pdf)