Vanquish Neo UHPLC System

A compact nano-flow UHPLC system

The Vanquish Neo UHPLC system delivers maximum performance 24/7 for reproducible nano-, capillary-, and micro-flow LC-MS with a pressure rating of 1500 bar, here is a link to Thermo's website if you wish to learn more: Vanquish Neo UHPLC System

  • Neo 1 (SN 8346660 and SN 8347102/ UW tag 2045623), Installed January/2023

System specifications

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    System pressure
  • up to 1500 bar (21,750 psi)
    Flow range
  • 1 nL/min – 100 μL/min, in 1 nL increments
  • 100 nL/min – 100 μL/min, recommended
    Injection volume range
  • 0.01–25 μL
    Injection volume precision
  • From 0.05 to 0.20 µL: ≤5.0% RSD
  • From 0.20 to 0.50 µL: ≤1.5% RSD
  • From 0.50 to 2.00 µL: ≤0.5% RSD
  • Above 2 µL: ≤0.25% RSD




Plate configuration

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Sample capacity (standard): 4 racks of 54 vials (≤ 1.5 mL) each
96-well plates with optional orientation barcoding to ensure the correct position in the autosampler and charger unit when loading and running the analytical sequence. Plates cannot be accepted in the wrong orientation.
Barcoded 96-well plate, V shaped well, 10-190 µl, 10pk 60180-217B (Fisher 03-255-015)
Barcoded 96-well plate, V shaped well, 10-190 µl, 100pk 60180-207B (Fisher 03-251-447)
Barcoded 96-well plate, U shaped well, 10-250 µl, 10pk 60180-216B (Fisher 03-255-014)
Barcoded 96-well plate, U shaped well, 10-250 µl, 100pk 60180-206B (Fisher 03-255-010)

You can also print/label the well plates yourself. Here are a couple of documents you can check out to start with:

HPLC setup

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the following document includes guidelines to setup the capillary trap/column on our LC-MS systems, this is intended as a supplement to the hands-on training required to use the UWPR instruments.

Instrument Method setup guidelines

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Detailed guide how to setup the VanquishNeo method see pdf

Thermo Video: Simple and Intelligent Method Creation

Setup the Gradient Back to Top
  • Note the buffer composition used on the VanquishNeo:
  • Buffer A: 0.1 % Formic Acid in Water (Fisher Water with 0.1% Formic Acid (v/v), Optima™ LC/MS Grade, LS118)
  • Buffer B: 0.1 % Formic Acid, 80% Acetonitrile in Water (Fisher Water with 0.1% Formic Acid (v/v), Optima™ LC/MS Grade, LS122500)
  • Formula 100% ACN to 80% conversion: % B [80%ACN] = 1.25 * % B [100%ACN]
Application Note: Improving Retention Time Precision and Chromatography of Early Eluting Peptides with Acetonitrile/Water Blends as Solvent B

%B [100% ACN] %B [80% ACN] %B [100% ACN] %B [80% ACN]
1 1.25 10 12.5
2 2.5 20 25
3 3.75 30 37.5
4 5 40 50
5 6.25 50 62.5
6 7.5 60 75
7 8.75 70 87.5
8 10 80 100
9 11.25 90 na
10 12.5 100 na


Installing VanquishNeo software SII for xcalibur

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Troubleshooting

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This video from Thermo describes how to Replace the Sample Loop, Needle Unit, and Needle Seat Vanquish HPLC/UHPLC Maintenance Videos: How to Replace the Sample Loop, Needle Unit, and Needle Seat

This video from Thermo describes how to Replace the Pump Head Vanquish HPLC/UHPLC Maintenance Videos: How to Replace the Pump Head

This video from Thermo describes how to Replace the Piston Seals and Support Ring Vanquish HPLC/UHPLC Maintenance Videos: How to Replace the Piston Seals and Support Ring

This video from Thermo describes how to Replace the Check Valves Vanquish HPLC/UHPLC Maintenance Videos: How to Replace the Check Valves

TIPS AND TRICKS: ISSUE 1: How to avoid and recover from autosampler blockages (pdf)

Convert bar to psi

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Formula for bar to psi conversion: 1 bar = 14.5038 psi or (bar x 14.5038 = psi) or 0.0689475729 bars in 1 psi.

To convert bar to psi follow this link: UnitConverters.net: Convert bar to psi

For a bar / psi chart follow this link: VICI tech support: Pressure Conversion Chart

Convert Bar to Psi Convert Psi to Bar
1 14.5 10 0.7
10 145.0 100 6.9
50 725.2 500 34.5
100 1450.4 1000 68.9
150 2175.6 1500 103.4
200 2900.8 2000 137.9
250 3626.0 2500 172.4
300 4351.1 3000 206.8
350 5076.3 3500 241.3
400 5801.5 4000 275.8
450 6526.7 4500 310.3
500 7251.9 5000 344.7
550 7977.1 5500 379.2
600 8702.3 6000 413.7
650 9427.5 7000 482.6
700 10152.7 8000 551.6
750 10877.9 9000 620.5
800 11603.0 10000 689.5
850 12328.2 11000 758.4
900 13053.4 12000 827.4
950 13778.6 13000 896.3
1000 14503.8 14000 965.3
1050 15229.0 15000 1034.2
1100 15954.2 16000 1103.2
1150 16679.4 17000 1172.1
1200 17404.6 18000 1241.1